Antigenic material for a chagas' disease detection system
US4870006A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 4, 1986 |
| Grant date | Sep 26, 1989 |
| Priority date | — |
| Expiry date | Nov 4, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/822
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
T. Cruzi polypeptide antigens that react with serum from chagasic individuals and does not cross-react with serum from uninfected individuals or individuals infected with related parasites such as Leishmania is described. The DNA from T. Cruzi culture trypomastigotes and epimastigotes coding for antigenic material having a molecular weight of 70 kd is identified, sequenced, and inserted into a cloning vector, which, in turn, is inserted into a host cell line. The expressed polypeptide is immunologically reactive with sera from Chagas' disease infect patients. The cloned gene for the 70 kd polypeptide is expressed and purified and a diagnostic test for Chagas' disease comprising the synthesized polypeptide is described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.