Method of obtaining gene product through the generation of transgenic animals
US4870009A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 15, 1983 |
| Grant date | Sep 26, 1989 |
| Priority date | — |
| Expiry date | Dec 15, 2003 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/89
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Mammalian genes that encode hormones are cloned and linked to strong promoter DNA sequences. The linked sequences are inserted in plasmids for amplification in prokaryotic cells, and multiple copies of the linked sequences are excised therefrom. Linked sequences are subsequently microinjected into fertilized eggs and the fertilized eggs are implanted into pseudo-pregnent females of the same species. As a result, transgenic animals are born having the linked sequences incorporated into their genomes and expressing the gene-encoded hormone. Because multiple copies of the linked sequences are frequently inserted and because production of the hormone is not limited to certain organs, as is the case with most endogenous hormones, the transgenic animals produce substantial amounts of the hormone. Hormone can be harvested from the living animal (and from its hormone-producing progeny) by extracting fluid, such as blood serum or ascites fluid, on a regular basis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.