Novel Host e. coli and use thereof
US4874697A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 29, 1986 |
| Grant date | Oct 17, 1989 |
| Priority date | — |
| Expiry date | Dec 29, 2006 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/19
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
E. coli deficient in protease activities, said activities being not inhibited with diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride, N-.alpha.-tosyl-L-lysyl chloromethyl ketone, N-tosyl-L-phenylalanine chloromethyl ketone, ethylenediamine tetra-acetic acid, leupeptin, antipain, .alpha..sub.2 -macroglobulin or chymostatin, and being inhibited with zinc chloride or copper chloride is disclosed. This E. coli is useful as a host for creating a transformant capable of expressing an exogenous protein or polypeptide. The protein or polypeptide expressed by the transformant may be extracted and purified with a quite low level of decomposition.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.