Device for solid phase sequencing of nucleic acid fragments

US4882127A · kind A · utility

Assignee

• Akademie der Wissenchaften der DDR · DE

Inventors

• Andre Rosenthal · Jena, DE
• Hans-Dieter Hunger · Panketal, DE
• Horst Kagelmaker · Panketal, DE
• Monika Gratschus · Berlin, DE

Key dates

• Filing date: Nov 12, 1987
• Grant date: Nov 21, 1989
• Expiry date: Nov 12, 2007

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/143333
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A device for solid phase sequencing of nucleic acid segments comprises a plurality of sequencing blocks for storaging and processing n nucleic acid fragments immobilized on carrier segments or in homogenous phase during a sequencing process, each of a sample dosing device for immobilizing n labeled nucleic acid samples from the sequencing blocks onto areas of a carrier matrix in a simultaneous fashion and including a capillary holder and a plurality of capillaries corresponding to the number n of different nucleic acid samples to be sequences and arranged according to the distribution of the reaction vessels with a distance between capillary orifices from the capillary holder greater than a depth of the reaction vessels, a plurality of carrier holders for fixing carrier matrices in a predetermined position during the immobilization of the nucleic acid samples with the sample dosing device and each having a recess corresponding to respective dimensions of the carrier matrix and a plurality of bores with a number and distribution pattern corresponding to those of the reaction vessels and with a diameter which is somewhat larger than a diameter of the capillary, a punch for simultaneo…