Use of volume exclusion agents for the enhancement of in situ hybridization
US4886741A · kind A · utility
121Cited by
0References
12Claims
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Assignee
Inventor
Key dates
| Filing date | Dec 9, 1987 |
| Grant date | Dec 12, 1989 |
| Priority date | — |
| Expiry date | Dec 9, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to methods for using volume exclusion agents to enhance in situ hybridization rates between short oligonucleotide probes and their target polynucleotides where the cells containing the target polynucleotides are adhered onto a glass substrate. In one aspect, the invention specifically relates to the use of volume exclusion agents to facilitate assay and diagnostic procedures for the detection of a virus, such as human papillomavirus (HPV). In addition, diagnostic kits embracing the above methods are described herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.