Preparation of polymeric thiol gels for covalent bonding of biologically active ligands
US4886755A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Oct 13, 1987 |
| Grant date | Dec 12, 1989 |
| Priority date | — |
| Expiry date | Oct 13, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Thiol gels are prepared from hydroxyl containing polymers by forming a 2-fluoro-1-methylpyridoxy derivative of the polymer followed by reacting the derivative with sodium dimethyldithiocarbamate and reducing to produce a sulfhydryl substituted polymer. Alternatively, the derivative is reacted with dithiothreitol to produce a sulfhydryl substituted polymer having free sulfhydryl groups spaced from the polymer. The thiol gel can be activated by means of 2,2'-dipyridyl disulfide and reacted with a free sulfhydryl containing biologically active ligand in order to provide an insolubilized ligand which can be used for various purposes including use as an immobilized biologically active material or use as a matrix in covalent chromatography.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.