Spiramycin resistance-conferring cloning vectors
US4886757A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 15, 1987 |
| Grant date | Dec 12, 1989 |
| Priority date | — |
| Expiry date | Apr 15, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/886
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A novel gene conferring resistance to spiramycin in Streptomyces and related organisms was cloned from a genomic library of Streptomyces ambofaciens DNA. A thirty-one Kb fragment of S. ambofaciens DNA including the spiramycin-resistance gene was isolated from this library on a cosmid designated pKC592. The novel spiramycin-resistance gene can be isolated on an .about.2.9 Kb BamHI fragment by subcloning restriction fragments obtained from the pKC592 insert DNA. This BamHI fragment contains all of the information required for the expression of the spiramycin resistant phenotype in Streptomyces. Vectors and transformants containing the novel spiramycin resistance gene are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.