RecA nucleoprotein filament and methods
US4888274A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 20, 1987 |
| Grant date | Dec 19, 1989 |
| Priority date | — |
| Expiry date | Jan 20, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A stable, single-stranded nucleoprotein filament adapted to complex specifically and stably with a target duplex DNA having a selected base sequence. The filament is composed of a single-stranded DNA probe having a region of homology with the target base sequence, and RecA protein bound stably to the DNA probe by adenosine 5'-(.gamma.-thio)triphosphate. The filament is useful in a novel system and method for enriching target duplex DNA which contains a region homologous to the probe sequence, for blocking selected restriction endonuclease sites in the target DNA, and in other DNA methodologies in which stable rapid triple-strand synaptic formation in duplex DNA can be exploited.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.