Method and apparatus for detecting low concentrations of (bio) chemical components present in a test medium using surface plasmon resonance
US4889427A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 11, 1988 |
| Grant date | Dec 26, 1989 |
| Priority date | — |
| Expiry date | Apr 11, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/805
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and an apparatus for detecting low concentrations of at least one (bio-) chemical component present in a test medium in a test cell, having a metal layer as sub wall with an external glass prism, using the surface plasmon resonance effect. A light ray is coupled in and, after attenuated total reflection, is coupled out and the intensity thereof is measured. The incidence angle position of the resonance curve is determined under the influence of the change, caused by the component, in the dielectric constant of the test medium near the metal layer. An adjustable selector is applied to the metal layer, in order to influence the incidence angle position of the resonance curve, through which the concentrations or concentration changes of one or more components in the test medium can be simultaneously determined through one or more differential measurements. A preferential association and therefor a higher concentration at the metal layer of one component above another is brought about.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.