Large DNA cloning system based on yeast artificial chromosomes
US4889806A · kind A · utility
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21Claims
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Key dates
| Filing date | Apr 15, 1987 |
| Grant date | Dec 26, 1989 |
| Priority date | — |
| Expiry date | Apr 15, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/942
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A large DNA cloning system is disclosed which is based on yeast artificial chromosomes. Cloning vectors are disclosed which allow the cloning of large segments of greater than 50 kb of exogenous DNA. The cloning vector comprises DNA sequences of an autonomous replication sequence, a centromere, a selectable yeast marker, two sequences that seed telomere function in vivo, and a cloning site within an interruptible yeast gene for insertion of the exogenous DNA segments.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.