Enzymatic electrode and electrode module and method of use
US4891104A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 24, 1987 |
| Grant date | Jan 2, 1990 |
| Priority date | — |
| Expiry date | Apr 24, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/001
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved enzymatic electrode and electrode module specifically adapted for use in a medical analyzer device is disclosed which permits rapid analysis of substances of interest contained within undiluted body fluids such as whole blood, serum and/or plasma. The enzymatic electrode is in fluid communication with an axially reciprocating probe which selectively transports either a quantity of a buffer aqueous solution or a calibrant aqueous solution presented at a wash cell or a body fluid specimen disposed within a sample cup to an active enzyme bearing membrane positioned adjacent the enzymatic electrode. The membrane comprises a composite membrane structure having a protective membrane layer adapted to prevent the passage of blood cells and other particulate or cellular substances therethrough as well as adjust the diffusion rate of the substance of interest desired to be measured into the membrane, an active enzyme layer adapted to convert the desired substance of interest to be measured into a detectable substance and a limiting membrane formed to prevent the passage of interfering low molecular weight substance therethrough. The membrane/reagent chemistry kinetics of the enzy…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.