Method for assaying primary amines, secondary amines and peptides using fluorogenic derivatization reagents
US4891323A · kind A · utility
1Cited by
4References
7Claims
0Family size
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Key dates
| Filing date | Mar 11, 1987 |
| Grant date | Jan 2, 1990 |
| Priority date | — |
| Expiry date | Mar 11, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/173845
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Primary amines, secondary amines and peptides are fluorometrically assayed using a fluorogenic derivatization reagent of the formula: ##STR1## where Z is an electron withdrawing group or a fused benzene ring, Y and Y', which are identical or different, are nitrogen or carbon and X is a halogen or other leaving group. The above fluorogenic reagent reacts with the primary amine, secondary amine or peptide to form a cyclic fluorescent adduct.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.