Erythrocyte agglutination assay

US4894347A · kind A · utility

Assignee

• Agen Limited · AU

Inventors

• Carmel J. Hillyard · Forsayth, AU
• Dennis Rylatt · Melbourne, AU
• Bruce E. Kemp · Melbourne, AU
• Peter G. Bundesen · Brisbane, AU

Key dates

• Filing date: Jan 13, 1988
• Grant date: Jan 16, 1990
• Expiry date: Jan 13, 2008

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/80
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

In an erythrocyte agglutination assay, the agglutination reagent comprises at least one erythrocyte binding molecule coupled to at least one specific analyte binding molecule wherein the erythrocyte binding molecule does not cause agglutination when incubated with erythrocytes in the absence of analyte. Preferably, the erythrocytes are endogenous to the blood sample to be tested, that is, a whole blood sample is assayed. Mixtures of conjugates and conjugates of analyte analogues with erythrocyte binding molecules may also be used as agglutination reagents. The reagents and their use in direct or indirect assays is disclosed.