Identification of NK cells and cytotoxic T lymphocytes
US4895796A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 6, 1988 |
| Grant date | Jan 23, 1990 |
| Priority date | — |
| Expiry date | Jan 6, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/809
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for distinguishing between NK cells and T lymphocytes is provided, which comprises contacting a sample containing lymphocytes with a first reagent comprising anti-CD3 and a first detectable label and a second reagent comprising a mixture of anti-CD16 and anti-GP160 (Leu-19) both labeled with a second detectable label, and identifying cells that react with the reagents, whereby cells that react with the first reagent are identified as T lymphocytes, and cells that react only with the second reagent are identified as NK cells. Cells that react with both reagents are identified as a unique subset of T lymphocytes some of which may mediate MHC unrestricted cytolysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.