Method for decontamination of mycoplasma-infected cell cultures

US4895803A · kind A · utility

Assignee

• BAYER HEALTHCARE LLC · DE

Inventors

• Gunter Hubner · Wuppertal, DE
• Helmut Brunner · Wuppertal, DE
• Hans-Joachim Zeiler · Velbert, DE

Key dates

• Filing date: Oct 22, 1986
• Grant date: Jan 23, 1990
• Expiry date: Oct 22, 2006

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N1/38
• WIPO field: Medical technology
• WIPO sector: Instruments

Abstract

A method for decontaminating cell cultures comprising contacting cell cultures with quinolone- and 1,8-naphthyridonecarboxylic acids of the formula (I) ##STR1## in which R.sup.1 represents methyl, ethyl, propyl, isopropyl, cyclopropyl, vinyl, 2-hydroxyethyl, 2-fluorethyl, methoxy, amino, methylamino, dimethylamino, ethylamino, phenyl, 4-fluorophenyl or 2,4-difluorophenyl, PA1 R.sup.2 represents hydrogen, alkyl having 1 to 4 carbon atoms, or (5-methyl-2-oxo-1,3-dioxol-4-yl)-methyl, PA1 R.sup.3 represents methyl or a cyclic amino group such as ##STR2## in which R.sup.4 represents hydrogen, alkyl having 1 to 4 carbon atoms, 2-hydroxyethyl, allyl, propargyl, 2-oxopropyl, 3-oxobutyl, phenacyl, formyl, CFCl.sub.2 --S--, CFCl.sub.2 --SO.sub.2 --, CH.sub.3 O--CO--S--, benzyl, 4-aminobenzyl or ##STR3## R.sup.5 represents hydrogen or methyl, R.sup.6 represents hydrogen, alkyl having 1 to 4 carbon atoms, phenyl or benzyloxomethyl, PA1 R.sup.7 represents hydrogen, amino, methylamino, ethylamino, aminomethyl, methylaminomethyl, ethylaminomethyl, dimethylaminomethyl, hydroxyl, or hydroxymethyl, PA1 R.sup.8 represents hydrogen, methyl, ethyl or chlorine PA1 X represents fluorine, chlorine or nitr…