Human gamma interferon-specific receptor protein, antibody against said protein, methods for obtaining said protein and said antibody and compositions containing said protein and antibody
US4897264A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 27, 1987 |
| Grant date | Jan 30, 1990 |
| Priority date | — |
| Expiry date | Mar 27, 2007 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Analysis of [.sup.125 I]. interferon gamma cross-linked to its receptor on various human cells by SDS-PAGE revealed that there are at least three different types of human interferon gamma receptors. In WISH, HeLa, FS11 and the other tissue cells an Mr 90,000-105,000 receptor was found. In monocytes and in the myeloid cell line KG-1 an Mr 140,000 receptor was found while in Daudi lymphoblastoid cells an Mr 95,000-115,000 receptor was found. The various receptors were isolated from these cells by extraction followed by affinity chromotography on an immobilized interferon gamma column. The resulting purified preparations retained their original affinity for interferon gamma and were used for immunizing mice and subsequent development of highly specific antibodies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.