Methods for purification of single-chain and double-chain tissue plasminogen activator
US4898825A · kind A · utility
17Cited by
1References
18Claims
0Family size
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Key dates
| Filing date | Apr 29, 1987 |
| Grant date | Feb 6, 1990 |
| Priority date | — |
| Expiry date | Apr 29, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/815
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A mixture containing a single-chain tissue plasminogen activator (tPA) and/or double-chain tPA is brought into close contact with a column carrying an immobilized Erythrina trypsin inhibitor as an affinity agent. Adsorbed protiens are eluted with eluents having different pHs with or without arginine or benzamidine, so that single-chain tPA is obtained in the eluent with a pH at least 4.5 and double-chain tPA is obtained in the eluent with a pH lower than 4.5.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.