Method for measuring cell counts and/or methane producing activity of methanogens
US4898833A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 16, 1987 |
| Grant date | Feb 6, 1990 |
| Priority date | — |
| Expiry date | Jun 16, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In the method for measuring cell counts or methane producing activity of Methanogens according the present invention, an extracted liquid is prepared by adding an addition solution for extracting a particular fluorescent substance inherent to Methanogens, for example, F.sub.420 exciting in the energy metabolism system of Methanogens in the object for examination and functioning as the electron carrier in the electron transport system or by heating the first object for examination. Since a particular fluorescent substance existing in the Methanogen cells in the first object for examination has been extracted out of the Methanogen cells, the intensity of fluorescence is increased upon measurement thereof. The particular fluorescent substance extracted from the inside to the outside of the Methanogen cells and water soluble substances already existing in the Methanogen cells are contained in the extracted and separated liquid obtained by applying solid-liquid separating procedures of removing solid particles greater than a predetermined grain size containing Methanogens from the extracted liquid. Then, the solid-liquid separating procedure is applied to the second object for examinati…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.