Electrophoretic method
US4900416A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 13, 1988 |
| Grant date | Feb 13, 1990 |
| Priority date | — |
| Expiry date | Sep 13, 2008 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An electrophoretic method comprises the steps of positioning a sample, which contains nucleic acid fragments having different numbers of bases, in an electrophoresis medium composed of an electrophoresis gel medium containing an aqueous poly(meth)acrylamide gel prepared by cross-linking polymerization of a (meth)acrylamide compound and a cross-linking agent in the presence of water and a compound having at least one carbamoyl group as a denaturing agent (or modifier), and applying pulsed electric fields to the electrophoresis medium in two directions, thereby to move the nucleic acid fragments in the presence of molecular sieve effects and to separate the nucleic acid fragments.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.