Chromophoric peptides for detecting peptidylglycine-.alpha.-amidizing monooxygenase

US4900658A · kind A · utility

Assignee

• Hoechst GmbH · DE

Inventors

• Wolfgang Konig · Hofheim, DE
• Hubert Mullner · Kelkheim, DE
• Jan Glauder · Frankfurt, DE

Key dates

• Filing date: Apr 9, 1987
• Grant date: Feb 13, 1990
• Expiry date: Apr 9, 2007

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/802
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A chromophoric peptide is prepared of the formula EQU DIS--D--Ala-Pro-R and salts thereof in which R represents Gly-OH, NH.sub.2 or Gly-R.sup.2 wherein R.sup.2 denotes a carboxyl protective group, and DIS denotes 5-dimethylaminonaphthalene-1-sulfonyl. The enzyme, peptidylglycine-.alpha.-amidizing monooxygenase, is quantitatively detected by contacting a sample containing the enzyme with the peptide of the above formula when R denotes Gly--OH, separating a resultant peptide of the above formula when R denotes NH.sub.2 and determining the amount thereof quantitatively. When R is Gly--R.sup.2, the protective group R.sup.2 is removed to produce the peptide when R is Gly--OH. Advantages of the peptide are that C-terminal degradation caused by carboxypeptidases is rendered more difficult by the incorporation of proline, and the peptide contains a fluorescent group.