Method for immunological determination of amines, monoclonal antibodies and kit of reagents for carrying out the method
US4900661A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Feb 12, 1986 |
| Grant date | Feb 13, 1990 |
| Priority date | — |
| Expiry date | Feb 12, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/868
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This method for immunological determination of amines in biological media consists of: (a) reacting a quinone (in particular, p-benzoquinone) with the amine (for example histamine) present in the test sample; (b) introducing into the medium a monoclonal antibody which specifically recognizes the amine-quinone reaction product formed; and (c) determining one of the forms of the monoclonal antibody present. When the quinone is used in the immobilized state, the monoclonal antibody present is determined in its complexed form with the amine-quinone reaction product (direct type method); when the quinone is used in the free state, the monoclonal antibody is determined in its free form (enzymoimmunometric method or competitive immunoenzymatic method).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.