Isolation of fibroblast growth factor
US4902782A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 7, 1988 |
| Grant date | Feb 20, 1990 |
| Priority date | — |
| Expiry date | Nov 7, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/849
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Basic Fibroblast Growth Factor (FGF) is substantially purified by the employment of affinity chromatography using heparin-linked support material. Described is a simplified three step procedure for extracting basic FGF from either mammalian brain or mammalian pituitary tissue. Salt precipitation, e.g., with ammonium sulfate is used to provide a partially purified precipitate that is then subjected to ion-exchange chromatography, e.g., using a Carboxymethyl-Sephadex column. Substantially pure basic FGF fractions are then obtained by fractionating the further partially purified fractions using affinity chromatography on a heparin-linked support e.g., Heparin-Sepharose.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.