Method for purifying a gene-expression product produced by recombinant DNA technique
US4902783A · kind A · utility
9Cited by
13References
5Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 15, 1987 |
| Grant date | Feb 20, 1990 |
| Priority date | — |
| Expiry date | Jun 15, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2770/24222
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
There is disclosed a method for purifying a gene-expression product produced by recombinant DNA technique which comprises a specific sequence of steps including adsorption treatment with silica gel, adsorption treatment with activated carbon, at least twice density gradient centrifugation and at least twice equilibrium density gradient centrifugation. The method of the present invention is very effective to remove allergen from gene-expression products contaminated therewith, enabling highly purified gene-expression products to be produced on a large scale.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.