Instrument for determination of the base sequence of nucleic acid
US4904366A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 4, 1987 |
| Grant date | Feb 27, 1990 |
| Priority date | — |
| Expiry date | Dec 4, 2007 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In the instrument for determination of the base sequence of the present invention, the ionic strength of a buffer solution in a gel is made lower at a detection part than in a region from the detection part towards a negative electrode. Therefore, at the detection part, the electric field intensity can be increased, resulting in a higher migration speed, and hence the electrophoretic pattern can be extended, so that the distance between two adjacent electrophoretic bands can be elongated. Consequently, according to the present invention, the slit width can be narrowed relatively to the electrophoretic pattern without actually narrowing the slit width, and the resolving power can be enhanced without lowering the detection sensitivity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.