Encoding antigens of M. Leprae

US4906742A · kind A · utility

Assignees

• Whitehead Institute For Biomedical Research · US
• Albert Einstein College of Medicine of Yeshiva University, a division of Yeshiva University · US
• The Board of Trustees of the Leland Stanford Jr. University · US

Inventors

• Richard A. Young · Weston, US
• Barry R. Bloom · Dobbs Ferry, US
• Ronald W. Davis · Menlo Park, US

Key dates

• Filing date: Jul 31, 1986
• Grant date: Mar 6, 1990
• Expiry date: Jul 31, 2006

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K39/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Genes encoding five immunodeterminant protein antigens of the leprosy parasite Mycobacterium leprae have been isolated. The gene encoding the M. leprae 65kD antigen was sequenced and a lambda gt11 gene sublibrary was constructed with fragments of the gene. Recombinant DNA clones producing specific antigenic determinants were isolated using monoclonal antibodies and the sequences of their insert DNAs were determined with a rapid primer extension method. Amino acid sequences for six different epitopes of the M. leprae protein were elucidated. A peptide containing sequences for one of these epitopes, which is unique to M. leprae, was synthesized and shown to bind the appropriate monoclonal antibody; The approach described here can be used to elucidate rapidly protein epitopes that are recognized by antibodies or T cells. In addition, the well-characterized M. leprae antigens can be used in prevention, diagnosis and treatment of leprosy.