Nucleic acid extraction methods
US4908318A · kind A · utility
42Cited by
2References
7Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Sep 4, 1987 |
| Grant date | Mar 13, 1990 |
| Priority date | — |
| Expiry date | Sep 4, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of extracting nucleic acids from a crude buffy coat fraction in an aqueous medium includes the steps of treating the buffy coat fraction to break open the white blood cells to release the proteins and the nucleic acids in the cells into the aqueous medium; solubilizing the proteins and the nucleic acids in the aqueous medium; and precipitating the nucleic acids under conditions under which the proteins remain in solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.