Virus-free in vitro assay for anti-HIV agents
US4910132A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 12, 1987 |
| Grant date | Mar 20, 1990 |
| Priority date | — |
| Expiry date | May 12, 2007 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/56988
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A virus-free assay to identify agents that interfere with the life cycle of HIV which comprises: (1) expressing in mammalian cells in the presence or absence of one or more of said agents, HIV gp120, the gene product of HIV tat, the gene product of HIV art/trs, all under the control of an HIV LTR promoter, and a control protein, not under the control of the HIV LTR promoter, such that infectious HIV particles are not produced; (2) quantitating the amount of the gp120 produced relative to the amount of control protein produced; and (3) comparing the relative amount of the gp120 produced in the presence of said one or more agents to the relative amount of the gp120 produced in the absence of said one or more agents. A plasmid capable of expressing the gene product of HIV art/trs in mammalian cells such that infectious HIV particles are not produced, which includes: (1) a DNA fragment comprising an HIV LTR promoter, and (2) and HIV cDNA restriction fragment carrying the art/trs gene but not the ATG start codon of the first coding exon for tat.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.