Method for minimizing interference by reductants when detecting cells in biological fluids
US4912035A · kind A · utility
9Cited by
8References
8Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 11, 1987 |
| Grant date | Mar 27, 1990 |
| Priority date | — |
| Expiry date | Jun 11, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the determination of cells in a sample is disclosed. The sample is typically urine and the method is useful in removing interfering reductants from the sample. The method comprises the steps of: PA0 (1) separating the cells from the sample, PA0 (2) washing the separated cells with: PA0 (a) an iron(III) chelate solution and PA0 (b) a non-ionic surfactant solution and PA0 (3) contacting the washed cells with a redox reagent so as to produce a detectable change due to the presence of the cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.