Extraction of granulocyte macrophage colony stimulating factor from bacteria
US4912200A · kind A · utility
2Cited by
3References
18Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 11, 1987 |
| Grant date | Mar 27, 1990 |
| Priority date | — |
| Expiry date | May 11, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/825
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of extracting granulocyte macrophage colony stimulating factor (GM-CSF) from GM-CSF-expressing bacterial cells comprising treating a suspension of GM-CSF-containing bacterial cells with an acid and an enhancing agent, removing substantially all of the suspension liquid from the cells, preparing a second suspension of the acidified cells, neutralizing said second suspension, and separating the GM-CSF containing liquid from the suspended cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.