Method of extending the linear range of an enzyme immunoassay by measuring off-peak absorbance
US4914023A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 5, 1987 |
| Grant date | Apr 3, 1990 |
| Priority date | — |
| Expiry date | Jun 5, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/818
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to enzyme immunoassays employing one or more enzyme label-substrate pairs which give rise to colored products and in which absorption by the product of substrate conversion by at least one enzyme label at a first wavelength, at or close to the optimum wavelength for absorbance, exceeds the linear range of the detector, which immunoassay includes the steps of measuring the said substrate conversion at a second wavelength at which absorbance by the relevant product is significantly lower than at the said first wavelength and calculating the true absorbance at the said first wavelength by utilizing the result of linear regression analysis of absorbance measurements obtained with product standards at the said first and second wavelengths within the linear range of the detector.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.