DNA-sequence encoding biosynthetic insulin precursors and process for preparing the insulin precursors and human insulin
US4916212A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 29, 1985 |
| Grant date | Apr 10, 1990 |
| Priority date | — |
| Expiry date | May 29, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/942
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Human insulin precursors containing the peptide chain B(1-29)-A(1-21) of human insulin and derivatives thereof with a bridging chain connecting the carboxyl terminus of the B(1-29)-chain with the amino terminus of the A(1-21)-chain are prepared by culturing a yeast host transformed with a replicable expression vehicle capable of expressing a DNA-sequence encoding the insulin precursor. The bridging chain is preferably relatively short and contains preferably from 2 to 8 amino acid residues. The bridging chain must not contain two adjacent basic amino acid residues (Lys or Arg) and has one Lys or Arg connected to the amino terminus of the A(1-21)-chain. Human insulin is prepared from the insulin precursors by in vitro conversion.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.