Process for detection of selected motile organisms
US4920063A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Sep 9, 1985 |
| Grant date | Apr 24, 1990 |
| Priority date | — |
| Expiry date | Sep 9, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/879
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and associated devices for detecting the presence of a particular motile organism within a sample are disclosed. The sample may be derived from dry milk, raw meat, poultry or a clinical specimen. A preferred method includes inoculating a selective enrichment medium containing a chemotactic attractant with the sample and contacting the selective enrichment medium with a nonselective motility medium containing a chemotatic attractant in a concentration less than the attractant concentration in the selective enrichment medium. Upon incubation, the motile organism metabolizes the chemotactic attractant, allowing the organism to move into the motility medium where it interacts with antibodies specific for the organism, thereby causing the formation of a persistent immobilization band. The methods are particularly useful in detecting Salmonella.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.