Monoclonal antibody-based immunoassay for cyclic DNA adducts resulting from exposure to crotonaldehyde or acrolein
US4923813A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 15, 1988 |
| Grant date | May 8, 1990 |
| Priority date | — |
| Expiry date | Jan 15, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/809
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Monoclonal antibodies specific for the 8R,6R- and 8S,6S-stereoisomers of 3-(2-deoxy-.beta.-D-erythropentofuransyl)-5,6,7,8-tetrahydro-8-hydroxy-6-m ethylpyrimido[1,2-a]purine-10(3H)one were produced. These cyclic 1,N.sup.2 -propanodeoxyguanosines are formed in DNA exposed to crotonaldehyde in vitro. Three of the four antibodies were most specific for one stereoisomer while the fourth was most specific for the other stereoisomer. Fifty % inhibition of binding in an enzyme-linked immunoabsorbent assay could be achieved with 0.2 picomol of either stereoisomer. A high-pressure liquid chromatography-enzyme-linked immunoabsorbent assay using two of these antibodies and capable of detecting 0.5 .mu.mol of 1,N.sup.2 -propanodeoxyguanosine per mol of deoxyguanosine was developed. The method was validated by comparison to results obtained with fluorescence assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.