Purification of hemoglobin and modified hemoglobin by affinity chromatography
US4925574A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 14, 1986 |
| Grant date | May 15, 1990 |
| Priority date | — |
| Expiry date | Mar 14, 2006 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention disclosed relates to a method for the purification of hemoglobin by the technique of affinity chromatography. Contrary to current belief, the binding of oxygen and selected polyanions to hemoglobin is not mutually exclusive. The novel method comprises immobilizing a polyanion which specicically binds hemoglobin via its polyanion binding site, on a chromatographic gel and passing the hemoglobin containing solution or mixture through the gel. The hemoglobin is thus retained in the gel, while impurities are eluted from the gel. The novel method is also applicable to the separation of unmodified hemoglobin from a liquid reaction mixture containing modified and unmodified hemoglobin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.