Methods of using chemiluminescent 1,2-dioxetanes
US4931223A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 30, 1988 |
| Grant date | Jun 5, 1990 |
| Priority date | — |
| Expiry date | Jun 30, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/973
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Processes are disclosed in which light of different wavelengths is simultaneously released from two or more enzymatically decomposable chemiluminescent 1,2-dioxetane compounds, said compounds being configured, by means of the inclusion of a different light emitting fluorophore in each of them, to each emit light of said different wavelengths, by decomposing each of said compounds by means of a different enzyme. Such processes can be used in multi-channel assays--immunoassays, chemical assays and nucleic acid probe assays--to detect the presence or determine the concentration of chemical or biological substances, and in multi-channel chemical/physical probe procedures for studying the microstructures of macromolecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.