Method for introducing desired agents into red blood cells
US4931276A · kind A · utility
Inventors
Key dates
| Filing date | Mar 13, 1989 |
| Grant date | Jun 5, 1990 |
| Priority date | — |
| Expiry date | Mar 13, 2009 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K35/18
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of introducing effectively non-anionic agents into mammalian red blood cells without unacceptable loss of cell contents, utilizing an osmotic pulse technique wherein an aqueous diluent medium is provided containing the desired effectively non-anionic agent(s) and an effective amount of water-soluble polyanion. The polyanion is non-disruptive to the lipid outer membranes of red blood cells and contains at least two anionic moieties per molecule. The method comprises suspending packed red blood cells in an aqueous solution containing a compound which readily diffuses into and out of the cells, rapidly creating a trans-membrane osmotic gradient by diluting the solution containing the cells in suspension with an essentially isotonic aqueous medium containing an effective amount of polyanion and one or more effectively non-anionic agents to be introduced, thereby causing diffusion of water into the cells with consequent swelling and increase in permeability of the outer membranes, and maintaining the increase in permeability for a period of time sufficient only to permit transport of the desired agent and polyanion into the cells and diffusion of the compound out of the cells. …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.