Process for recovering microbially produced interleukin-2
US4931543A · kind A · utility
26Cited by
22References
17Claims
0Family size
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Key dates
| Filing date | Mar 25, 1988 |
| Grant date | Jun 5, 1990 |
| Priority date | — |
| Expiry date | Mar 25, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/825
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for recovering substantially pure rIL-2 from transformed microorganisms in which the cells are disrupted, impure insoluble rIL-2 is separated from the bulk of the cellular components, the separated impure rIL-2 is solubilized and partially purified in a reduced form, the solubilized rIL-2 is oxidized, the oxidized rIL-2 is purified to clinically acceptable levels, and the oxidized, purified IL-2 is denatured by placing it into a solution of a chaotropic agent, solids are removed from the solution and rIL-2 is renatured from the solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.