Particle separation method
US4935147A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 26, 1988 |
| Grant date | Jun 19, 1990 |
| Priority date | — |
| Expiry date | Oct 26, 2008 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54333
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is disclosed for separating a substance from a liquid medium. The method comprises combining the liquid medium containing the substance with magnetic particles under conditions for non-specific chemical binding of the magnetic particles. Thereafter, the medium is subjected to a magnetic field gradient to separate the particles from the medium. The preferred non-specific binding is achieved as the result of charge interactions between the particles usually by means of a polyionic reagent. The method of the invention has particular application to the separation of cells and microorganisms from aqueous suspensions and also to the determination of an analyte in a sample suspected of containing the analyte. The analyte is a member of a specific binding pair (sbp). The sample is combined in an assay medium with magnetic particles and a sbp member complementary to the analyte. Magnetic or non-magnetic particles capable of specific binding to the analyte or its complementary sbp member must be included in the assay medium. The combination is made under conditions for non-specifically aggregating the magnetic particles or coaggregating the magnetic and non-magnetic particles when n…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.