Process for producing tissue plasminogen activator
US4935368A · kind A · utility
7Cited by
2References
5Claims
0Family size
Assignees
Inventors
Key dates
| Filing date | Jun 27, 1986 |
| Grant date | Jun 19, 1990 |
| Priority date | — |
| Expiry date | Jun 27, 2006 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/21069
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention discloses a process for producing tissue plasminogen activator. The process comprises cultivating Escherichia coli, yeast, or mammalian cells into which the vector having the gene for human tissue plasminogen activator (tPA) has been introduced by the recombinant DNA technology. In the cultivation or purification process, a specific antiplasmin agent, which is one of the protease inhibitors, is added to the medium and/or the buffer solutions used for purification, so as to prevent the conversion of single-chain form tPA into double-chain form tPA by a protease which is present in the medium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.