Art nucleotide segments, vectors, cell lines methods of preparation and use
US4935372A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 20, 1986 |
| Grant date | Jun 19, 1990 |
| Priority date | — |
| Expiry date | May 20, 2006 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/221
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A gene and gene product that regulates the expression of the capsidal envelope genes of HTLV-III/LAV and that can be used to regulate the expression of heterologous (non-viral) genes as well is disclosed. This art gene consists of two exons and can be used in creating nucleotide segments, vectors and cell lines. A new method for screening for compounds that inhibit the replication of HTLV-III is also described and comprises: PA0 (1) transfecting a T-cell line with the HTLV-III art and env genes; PA0 (2) thereafter, adding a preselected compound to the transformed cell line in increasing concentrations; and PA0 (3) determining whether the compound effects the art function without being toxic to the cell. An additional parameter to use in diagnosis of AIDS disease is also described. The use of the art gene and gene product in AIDS therapy is also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.