Polycationic buffers and method for gel electrophoresis of nucleic acids
US4936963A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 23, 1989 |
| Grant date | Jun 26, 1990 |
| Priority date | — |
| Expiry date | May 23, 2009 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides for polycationic buffers for the gel electrophoresis of and methods for obtaining improved separation and resolution of nucleic acids. In particular, the invention provides for a buffer consisting essentially of histidine for the gel electrophoresis of oligonucleotides ranging from a few to about 70 nucleotides in length. Polycationic buffers may be used for both analytical and preparative purposes and result in a nearly two-fold improvement in both separation and resolution of the nucleic acid bands. Furthermore, the use of polycationic buffers reduces the electrophoresis time by a factor of about three due to the low conductivity of the buffers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.