Capillary separation system with electric field assisted post separation mixing
US4936974A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 3, 1988 |
| Grant date | Jun 26, 1990 |
| Priority date | — |
| Expiry date | Nov 3, 2008 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44721
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A capillary zone electrophoresis (CZE) system provides for rapid, non-turbulent post-separation diffusional mixing of sample effluent with a fluorogenic-labelling reagent permitting sensitive detection of well-defined sample component zones. A separation capillary extends into a mixing capillary so as to define an annular gap therebetween. The effluent of the separation capillary is mixed with the labelling reagent, which is introduced through the annular gap. A power supply and opposing electrodes establish an electric field which induces electro-osmotic flow of the sample and charge-related differential electrophoretic migration to define component zones. The electric field also causes the separation capillary effluent to diverge as it issues into the mixing capillary so as to facilitate diffusional mixing with the reagent fluid flow without causing significant turbulence. Thus, fluorescence labelling is effective with minimum zone broadening. This system combines the high resolving power of CZE separation with the sensitivity of labelled fluorescence detection to attain an improved system for analyzing biological samples.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.