Method for the determination of bilirubin in solution
US4937186A · kind A · utility
3Cited by
8References
8Claims
0Family size
Inventors
Key dates
| Filing date | Jan 28, 1988 |
| Grant date | Jun 26, 1990 |
| Priority date | — |
| Expiry date | Jan 28, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/26
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A novel analytical method for bilirubin in biological fluids. Bilirubin is catalytically oxidized to biliverdin in the presence of bilirubin oxidase (BOX) and the latter is revertedly back-converted to initial bilirubin in the presence of suitable reducing agents. The corresponding oxidation of said reducing agents provides a signal which is monitored and provides the desired analytical data. When using selected organic fluoro-compound, said signal is provided by the release of F.sup.- ions which can be measured electrometrically.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.