Lateral flow, non-bibulous membrane assay protocols
US4943522A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 10, 1988 |
| Grant date | Jul 24, 1990 |
| Priority date | — |
| Expiry date | Aug 10, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/81
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and apparatus for conducting specific binding pair assays, such as immunoassays, is described. A porous membrane capable of non-bibulous lateral flow is used as assay substrate; a member of the binding pair is affixed in an indicator zone defined in the substrate. The sample is applied at a position distant from the indicator zone and permitted to flow laterally through the zone; any analyte in the sample is complexed by the affixed specific binding member, and detected. A novel method of detection employs entrapment of observable particle in the complex. Blood is a particularly preferred sample as the red blood cells can be used as the observable particles for detection of the complex.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.