Electrochemical methods of assay
US4945045A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 23, 1988 |
| Grant date | Jul 31, 1990 |
| Priority date | — |
| Expiry date | Jun 23, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/806
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A ligand in a sample can be assayed using electrochemical apparatus containing a working electrode and components comprising: PA1 (a) the sample, PA1 (b) a specific binding partner to the ligand, or a specific binding partner to the ligand and selected from ligand analogues and specific binding partners, and PA1 (c) an electron-source or electron-acceptor; at least one of components (b) being labelled with an electron-transfer mediator capable of aiding the transfer of electrons between the electron-source or electron-acceptor and the working electrode by accepting electrons from the electron-source and donating them to the working electrode, or accepting the electrons from the electrode and donating them to the electron-acceptor. The method includes the step of determining whether, and, if desired, the extent to which, the said transfer of electrons is perturbed by at least one of (i) formation of a complex of said ligand with a specific binding partner and (ii) controlled external influence which produces a perturbation of said transfer of electrons as a function of said complex formation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.