Modification of cellulose normally synthesizied by cellulose-producing microorganisms
US4950597A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 25, 1988 |
| Grant date | Aug 21, 1990 |
| Priority date | — |
| Expiry date | May 25, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/823
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention involves a process for screening for and isolating spontaneously occurring or induced cellulose II-producing microorganisms. The process comprises a series of steps in various embodiments. Initially, cellulose-producing microorganisms from a first culture are plated out on a nutrient agar plate. The nutrient agar plate is then incubated to facilitate formation of colonies from single microorganisms. Samples of liquid nutrient medium are then inoculated with microorganisms from colonies having a smooth configuration, as compared to the usual rough colony configuration. The inoculated samples are then aerobically incubated to facilitate microorganism proliferation and pellicle formation. From these incubated samples are selected microorganisms, which, after a cultivation period, have proliferated but not formed a pellicle. Said selected microorganisms produce cellulose II instead of the cellulose I produced by pellicle-forming organisms.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.