Method for exchanging homologous DNA sequences in a cell using polyoma encapsulated DNA fragments
US4950599A · kind A · utility
Inventor
Key dates
| Filing date | Jan 29, 1987 |
| Grant date | Aug 21, 1990 |
| Priority date | — |
| Expiry date | Jan 29, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/948
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for altering a cell by exchanging a preselected cellular DNA sequence with an exogenous DNA sequence different from the cellular DNA sequence employs an exogenous DNA sequence encapsidated in a polyoma or polyoma-like capsid. The polyoma capsid is then contacted to the cell so that the exogenous DNA sequence is introduced within the cell and exchanges with the preselected cellular DNA sequence by homologous recombination. A preferred article of manufacture comprises a polyoma capsid and a plurality of DNA sequences encapsulated within the polyoma capsid. The DNA sequences each comprise not more than an incomplete portion of a single preselected gene. The exogenous DNA sequence may optionally be complexed to a DNA binding protein, such as a recA protein, prior to encapsulating the exogenous DNA sequence within a viral capsid, so that the uptake of the DNA sequence into the capsid is enhanced.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.