Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from Streptomyces lipmanii
US4950603A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 2, 1987 |
| Grant date | Aug 21, 1990 |
| Priority date | — |
| Expiry date | Nov 2, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/76
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
DNA compounds encoding the Streptomyces lipmanii isopenicillin N synthetase (IPNS) gene are useful for constructing a variety of recombinant DNA vectors. The vectors are useful in producing IPNS in a wide variety of host cells, such as Streptomyces, Penicillium, and Cephalosporium. DNA compounds encoding the transcription and translation activating sequence and transcription termination sequence of the S. lipmanii IPNS gene are also useful in the construction of expression vectors, especially Streptomyces expression vectors. The S. lipmanii IPNS gene can be isolated from plasmid pOGO239, available from the Northern Regional Research Center under accession number NRRL B-18250.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.