Polymers derived from crosslinked polystyrenes and dextrans, their methods of preparation and their applications for the analysis and purification of molecules of biological origin
US4950712A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 22, 1988 |
| Grant date | Aug 21, 1990 |
| Priority date | — |
| Expiry date | Aug 22, 2008 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/545
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
These resins derived from a crosslinked styrene (co)-polymer, or from a crosslinked dextran, comprise a (co)-polymer chain which is substituted with one or more groups, which may be identical or different, belonging to the following categories: --Z--A.sub.1 ; --Z--A.sub.2 ; --Z--A.sub.1 --Z'--A.sub.2 ; --Z--A.sub.1 --A.sub.3 --A.sub.2 ; --Z--A.sub.1 --A.sub.4, where Z=spacer chain, Z'=linking chain, A.sub.1 =phosphate residue, A.sub.2 =residue of purine or pyrimidine base, A.sub.3 =sugar residue and A.sub.4 =residue of a molecule participating in the polar structure of various phospholipids. These resins are applicable for the analysis and purification of molecules of biological origin, in particular as a stationary phase in ion-exchange and affinity chromatography, especially for carrying out the fractionation of protein mixtures. Their interaction with proteins, in particular the anti-DNA and anti-phospholipid antibodies present in the serum of patients suffering from SLE, makes them applicable to operations for the selective purification of the different types of antibodies developed by lupus patients.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.