Hydrolyzable compounds which release electron transfer agents and analytical use of same
US4952495A · kind A · utility
21Cited by
11References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 8, 1987 |
| Grant date | Aug 28, 1990 |
| Priority date | — |
| Expiry date | Jun 8, 2007 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/924
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Hydrolyzable substrates comprise blocked moieties which, when cleaved from the substrate during hydrolysis, provide electron transfer agents. The released electron transfer agents can be recycled between a reductant and a reducible compound that upon reduction provides a detectable species. Alternatively, they can be recycled between an oxidant and an oxidizable compound that upon oxidation provides a detectable species. These substrates are useful in analytical compositions, elements and methods for the determination of hydrolytic analytes, such as hydrolytic enzymes or biological cells containing such enzymes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.